Helix Tools

CRISPR, prime editing, and PCR utilities for real experiments.

Run plans, sanity-check primers, and export ready-to-share PDFs. Fast, reproducible, and built for lab teams.

GPU-awareIn-silico firstWet-lab ready
Planner · CRISPR / PrimePopular · Start here

CRISPR / Prime Edit Planner

Paste your target and guides to get geometry, distances, RT/PBS helpers, diagrams, and a printable PDF plan validated against published designs.

Open planner
PCR · Product scanQuick PCR sanity check

PCR Product Finder

Scan primer pairs, see GC/Tm and amplicon sizes instantly. Perfect for fast pre-order sanity checks and printable reports.

Open PCR finder
Planner · BatchMulti-edit

Batch Edit Planner

CSV → multiple edit plans at once. Perfect for guide/edit screening campaigns.

Plan in batch
PCR · BatchCSV / multi

PCR Batch Finder

Paste primer pairs and scan multiple amplicons; export CSV of product counts and flags.

Run PCR batch
Primers · QualityClamp & Tm

Primer Quality Checker

GC%, Tm, 3' clamp, poly-runs, and dimer heuristics. Sanity-check single primers or pairs before PCR or synthesis.

Check primers
Primers · BatchOrdering prep

Batch Primer Checker

Paste a list of primers to get GC, Tm, and flags in one table before you place an order.

Run batch
Saved · Read-onlyShareable

Saved Plans

Browse shareable, read-only plans you’ve saved on the server.

View saved

Console

Quick sequence console

Reverse complement, GC%, translation — no fuss.

GC%: liveLength: liveFrame 0 translation

Sequence console

GC% 0.0Length 0 ntFrame 0 translation

Cleaned

Reverse complement

GC%

0.0%

Translation (frame 0)